Elucidating the behavior of an enzyme

Posted by / 04-Sep-2018 09:58

Elucidating the behavior of an enzyme

If the aim is to understand the physiological role of an enzyme it needs to be studied in conditions that do not depart more than necessary from physiological conditions.All this is simply common sense, but it is useful to consider it in a little more detail.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.How should kinetic parameters be deduced from kinetic measurements?What information needs to be provided in reporting the results of a kinetic experiment in such a way that they can be confirmed by other workers?It is also easy to illustrate the consequences of violating this requirement in a linear regression.Virtually none of the equations considered in enzyme kinetics lead to linear models if properly analysed, but in practice it is not difficult to ensure that the independent variables are indeed independent even in a non-linear regression: in essence, it means that knowledge of the values of one independent variable must not allow the values of another to be calculated.

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